Abstract

Frozen sections of turkey pituitary glands were processed for Confocal Láser Scanning Microscope (CLSM) observation. The double-labeling method was appüed to those frozen samples. Sections were first incubated with a primary antibody (either rabbit anti-turkey growth hormone, GH or rabbit anti- turkey prolactin, PRL) and then incubated with Texas red anti-rabbit antiserum. After antibody incubations were completed, the sections were prepared for labeling with fluorescent ceramide. Fluorescent imaging was performed using a CLSM, it reveáis not only the distribution of GH and PRL cells but also the spatial relationship of hormone granules and Golgi complex.