Plants Related To Early Evolutionary Events (Bryophytes) Contain Lacandonia Granules Previously Discovered In Flowering Plants
The early evolution of the plant kingdom is controversial, and bryophytes are argued to be the earliest divergent plants. Indeed, the origin and diversification of land plants marks an interval of unparalleled innovation in the history of plant life. Previous ultrastructural analysis of the nucleus revealed the presence of Lacandonia granules. Cytochemical, immunocytochemical and in situ hybridization studies suggested that Lacandonia granules are involved in mRNA storage. In addition, Lacandonia granules have been located in other flowering plants of the order Triuridales (such as Triuris alata) and also in non-flowering plants. Therefore, in order to understand the evolution of RNA processing, we demonstrated the presence of Lacandonia granules in three species of non-vascular plants (Bryophytes). In this study, we analyzed three species of Bryophytes using transmission electron microscopy and found granules in sporophyte cell nuclei of all species. Moreover, the presence of few granules in the interchromatin and perichromatin space was a constant feature of all nuclei of Bryophytes. Thus, non-abundant particles of around 32 nm in diameter were observed in the perichromatin and interchromatin space. Perichromatin fibers are usually present in continuity with granules, forming a fibrogranular environment. Finally, we demonstrated that these particles are positive after the EDTA regressive technique preferential for ribonucleoproteins. In summary, this study suggests that Lacandonia granules contribute to the understanding of the spatiotemporal organization of several mRNA processing factors in the nuclear subcompartments and verifies the conservation of the event throughout the evolutionary process in the Plant Kingdom.