Abstract

A rapid protocol for quick fixation of eukaryotic cell suspensions for routine transmission elec­trón microscopy is presented. Duration of the fixation, washing, and dehydration steps is extremely reduced so that this pre-embedding process can be accomplished in about 60 minutes. The methodology was successfully applied to different single-cell suspensions, such as trypanosomatid protozoa and mice peritoneal cells. Ultrastructural observation showed that the fine morphology of the samples could be finely preserved.