TEM Observation of Thin and Thick Tissue Sections and Comparison of Respective Images of the Golgi Structure
Abstract
The structure and functional activity of the Golgi complex in cells of the chicken pituitary gland were studíed by preparing specimens in plástic sections with a thickness of 70 nm and of 1.0 um. The thin sections were observed with TEM operated at 120 kV while thick sections were observed at 200 kV. Complimentary information regarding ofthe Golgi complex is obtained when images of thin and thick tissue sections were observed. Images of thin sections show that developing granules are limited to inside the Golgi cisternae while mature granules are free in the cytoplasm. Stereo images of thick sections show that in those cells which are active in synthesis, the Golgi complex extends throughout the cytoplasm, hormonal granules of different sizes are being formed and stored in different parts of Golgi complex. Few granules are free in the cytoplasm, being principal 1 y inside Golgi cisternae. The structural comparsion between stereo images obtained from thin and thick tissue sections is valuable for better interpretation of intracellularorganelle configuration.