The understanding of the interaction between graphene with chitinous polymers remains a challenge. Herein, we propose the extraction of partially deacetylated chitin from Litopeneaus Vannamei shrimp’s waste (CH30) to produce a nanocomposite film with graphene and compared it with a high deacetylated chitosan (CH75). Structural characterization was carried out by X-ray Photoelectron Spectroscopy (XPS), Fourier Transform Infrared Spectroscopy (FTIR), Raman, Scanning Electron Microscopy (SEM), and Thermogravimetric Analysis (TGA-DTG). Our findings reveal the successful functionalization of graphene with chitin to form a nanocomposite film. These findings shed light on the interaction between graphene and chitinous polymers in regards to give an additional value to shrimp shell waste.