The aim of this work is to show options for preparing, observing, recording images of biological samples and provide recommendations on optimal procedures for each specimen. Some basic concepts are developed to take into account when preparing and observing biological samples of different origin. Some experiences carried out in the Scanning Electron Microscopy Service of UNNE are described. The procedures to observe, analyze and record morphological details of different
biological sample types are explained. According to the fragility of the sample, some working conditions are recommended.